FastQCFastQC Report
Sun 14 Apr 2019
SRR6576769_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576769_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137837
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3530.2560995958995045No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3030.21982486560212425No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2030.14727540500736377No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1860.1349419967062545No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1670.12115759919325No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1650.1197066099813548No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1640.11898111537540718No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1610.11680463155756438No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA1460.10592221246835029No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1440.1044712232564551No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAA450.001011194241.774875
GTATTTA500.001685339137.6520351
GTTTAGT500.001685339137.6520351
TAGTACT500.00169741437.5973854
TCCTAGG651.4076209E-436.1513332
GTATCAA5550.035.616791
TTAAGCA550.002709679534.1794434
TATTACA550.002709679534.1794434
CTAGGTA550.002709679534.1794434
TTTATTC851.7444803E-533.1741643
TGGGAGT600.004149812331.3311586
TCAACGC6750.030.6349094
ATCAACG6800.030.409653
AACGCAG6850.030.1876856
TTATTCT953.7303937E-529.6821484
CAACGCA7100.029.1247375
GTACTTG856.7307893E-427.6853181
TATCAAC7400.027.3089122
ATCCTAG700.00875029326.8943121
GCTCGAT350.00882757426.85527664-65