FastQCFastQC Report
Sun 14 Apr 2019
SRR6576771_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576771_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426525
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11320.26540062130004105No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8830.20702186272785883No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8100.18990680499384563No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7720.18099759685833186No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7440.17443291717953227No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6500.15239434968641932No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6120.14348514155090558No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5880.13785827325479164No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5850.13715491471777738No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5280.12379110251450676No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5200.12191547974913546No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5050.11839868706406424No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4860.11394408299630737No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4820.11300627161362171No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4750.1113651016939218No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4660.10925502608287907No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC4640.10878612039153625No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG4380.10269034640407948No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG4310.10104917648437958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13800.053.8080371
TCAACGC17200.042.068754
ATCAACG17400.041.5851973
AACGCAG17750.041.294636
CAACGCA17900.040.6860925
GCAAACG704.6304813E-640.283051
CGCAGAG20250.035.9644978
ACGCAGA20300.035.8759167
GTACATG23750.035.4208151
TATCAAC21250.034.9394762
TACATGG24350.034.5439722
ACATGGG25250.033.3087963
CATGGGA11200.031.4637554
GCAGAGT23850.030.7328979
ATGGGTA4150.030.5691225
GGTATCA6100.029.276751
GTATACG650.006151872728.9211641
CCCTACG650.006158925628.9143859
CATGGGT6900.028.6000984
ATACCTT1406.369246E-726.8490726
Produced by FastQC (version 0.11.2)