FastQCFastQC Report
Sun 14 Apr 2019
SRR6576771_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576771_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426525
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11410.26751069691108376No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8760.20538069280815896No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7760.18193540824101753No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7300.17115057734013248No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6630.1554422366801477No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6160.14442295293359123No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6130.143719594396577No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5610.13152804642166344No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5580.1308246878846492No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5570.1305902350389778No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5290.12402555536017819No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5160.1209776683664498No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4860.11394408299630737No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC4810.1127718187679503No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4620.10831721470019341No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4580.10737940331750775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4320.101283629330051No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG4290.10058027079303675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13250.052.574971
GGTATCA5350.043.1097531
ATGGGTA4950.037.9729775
GTACATG27050.037.7594531
GCGTTAA250.001702302537.59765658-59
TACATGG26900.037.2089272
CATGGGT7400.036.8312234
ACATGGG27400.036.1869283
ATCAACG19100.035.6742173
AACGCAG19050.035.5211756
TCAACGC19050.035.5211754
GGTACCT4900.035.50848
CAACGCA19200.034.998925
GGGTACC5050.033.498937
TGGGTAC5300.032.8054286
CATGGGG10650.030.8864694
ACGCAGA22250.030.4125167
CATGGGA10400.030.2734094
CGCAGAG22650.030.1040738
TATCAAC23350.029.5835512
Produced by FastQC (version 0.11.2)