FastQCFastQC Report
Sun 14 Apr 2019
SRR6576772_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576772_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586097
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14350.24484001794924734No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11120.18972968638297075No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10380.17710378998698167No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9150.15611750273418903No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA8520.1453684287754416No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7590.12950074816967155No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7050.1202872562050309No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC6660.11363306756390153No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6620.11295058667763186No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6400.10919694180314864No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6290.107320119365907No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG6150.10493143626396315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13800.052.5867921
GGTATCA5700.039.6827161
GTACATG32800.038.0721171
TACATGG33100.037.4884832
ACATGGG33250.036.471193
CATGGGT9400.035.9989244
ATCAACG20000.035.956993
CAACGCA20250.035.2779585
AACGCAG20850.034.9396
TCAACGC20450.034.932944
ATGGGTA5950.034.755265
CGCGTTA550.002716326434.18079494
GTAATAG1658.367351E-1131.4154831
CATGGGA15150.030.7119524
TGGGTAC5600.030.2133796
TATCAAC24050.030.0973012
GGGTACC5550.029.6387547
TACACCG804.778895E-429.374125
ACGCAGA25400.028.3101777
CGCAGAG25850.028.1809738
Produced by FastQC (version 0.11.2)