FastQCFastQC Report
Sun 14 Apr 2019
SRR6576773_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576773_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553683
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13530.2443636521258554No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10040.18133119492561628No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9700.17519049708948983No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8930.1612836225782623No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA8910.16092240505849015No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7590.13708204875352864No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7200.13003830711797185No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC6430.11613143260674429No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6350.11468656252765573No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG6260.11306108368868108No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6160.11125499608982034No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT6160.11125499608982034No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5950.10746221213221285No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5930.10710099461244069No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5770.10421125445426353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14700.057.884711
ATCAACG20200.042.33383
TCAACGC20250.041.9972464
AACGCAG20550.041.6127856
CAACGCA20850.040.788695
GTACATG32550.035.8181651
TACATGG32000.035.6992382
TATCAAC24350.034.9447752
ACTAATA1107.667768E-834.1900672
ACATGGG33200.033.9656873
ATGGGTA6100.033.8914455
ACGCAGA25400.033.6670387
CGCAGAG25650.033.522088
CATGGGT8600.032.7808384
CTAGTAC1457.4396667E-1032.4040453
GGTACCT6100.030.0401448
GTATTAA1751.70985E-1029.5499841
TCTATGC1751.72804E-1029.5339723
TACCCCG804.7861386E-429.3661675
TGGGTAC6400.029.3661676
Produced by FastQC (version 0.11.2)