FastQCFastQC Report
Sun 14 Apr 2019
SRR6576774_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576774_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596535
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12360.20719656013477836No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10050.16847293117755036No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8990.15070364689414703No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8340.13980738766375822No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA8040.13477834494204027No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7470.12522316377077625No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6610.11080657463518487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14700.056.290231
ATCAACG21350.038.9643483
CAACGCA21600.038.5133745
AACGCAG22050.038.1536836
TCAACGC21850.038.072724
TAGCGCG500.001701482637.59954
TACATGG33050.033.998842
GTACATG32900.033.8680461
TATCAAC25650.032.6264882
ACATGGG33300.032.462033
ACGCAGA26100.031.8731387
CGCAGAG26050.031.5734758
CATGGGA14800.031.4387664
CCGAACT902.5861526E-531.3434221
GGTATCA7300.030.9140621
CATGGGT9000.028.1996254
GCAGAGT30300.027.1448529
CCCGCTA700.00883325426.8567833
AGAGTAC27750.024.81227910-11
ATGGGAG8400.024.05925
Produced by FastQC (version 0.11.2)