FastQCFastQC Report
Sun 14 Apr 2019
SRR6576774_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576774_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596535
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11840.1984795527504673No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11430.1916065276974528No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8770.14701568223155387No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7740.12974930222032235No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7010.11751196493080876No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6990.11717669541602756No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6280.10527462764129514No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6230.10443645385434215No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG6230.10443645385434215No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6100.1022572020082644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14300.057.689461
ATCAACG21050.038.6289063
TCAACGC21800.037.9467474
AACGCAG22400.036.9303176
CAACGCA22550.036.8930975
GTACATG31400.036.3312381
GGTATCA6050.035.842391
TACATGG32200.035.470622
ACATGGG32100.035.141853
TATCAAC25950.031.8781932
ACGCAGA26750.030.9222347
CGCAGAG26750.030.7465388
CATGGGA13950.029.6501464
ATCCGCG650.00615292528.922027
GCAGAGT30650.026.9875959
CATGGGG14650.026.9500794
CATGGGT8250.026.2072984
ATGGGTA5600.026.0190875
GGGTACC4700.024.9990847
AGAGTAC27900.024.67830810-11