FastQCFastQC Report
Sun 14 Apr 2019
SRR6576775_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576775_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543222
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11690.21519746991101243No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9690.17838010978936789No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA8350.153712478507866No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8330.15334430490664958No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7440.13696057965251776No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6670.1227858960056846No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6170.11358155597527347No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5470.10069547993269787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16100.057.5086981
CGTAGAC250.005232872456.4045872
CCCGCTA708.610732E-846.990843
GCAAACG1007.039489E-1042.2995451
ATCAACG22550.040.6350943
TCAACGC23400.038.9582184
AACGCAG23600.038.8271756
CAACGCA23550.038.710085
ACGCAGA26500.034.4008457
CGCAGAG26900.033.8893058
CCGCTAA702.1859915E-433.5648884
TATCAAC27750.033.1990932
TGCCCGC1755.456968E-1232.228221
GGTATCA6250.030.0796761
GTACATG32300.029.9749091
GCAGAGT30700.029.6918039
TACATGG31950.029.4233632
ACATGGG31850.029.2125153
GCGGTAT1452.654997E-829.1720981
TACACCG1005.325504E-528.1945045