FastQCFastQC Report
Sun 14 Apr 2019
SRR6576775_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576775_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543222
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11710.2155656435122289No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11620.21390886230675488No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7940.14616491968292888No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7700.14174683646833156No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7120.13106980203305463No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6920.12738806602089017No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6320.1163428579843968No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG6220.11450198997831457No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5920.1089793859600679No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5860.10787486515641855No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5830.10732260475459388No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5590.10290452153999653No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5550.10216817433756364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15600.057.6692961
TATCACG450.001014201141.7717672
ATCAACG21150.041.105193
AACGCAG22750.038.6274036
TCAACGC22850.038.25274
CAACGCA22750.038.2142755
GTACATG29250.035.7489241
TACATGG29950.034.676142
ACATGGG30850.033.664523
TATCAAC26550.033.629812
ACGCAGA26050.033.012527
CGCAGAG26100.032.610198
CATGGGG14150.028.8933684
GCAGAGT29950.028.4182289
GGTATCA6800.027.0141661
CATGGGA12000.026.62954
AGAGTAC28100.025.93824810-11
GTCTTAG1501.1389948E-625.1208671
CATGGGT8800.024.5646464
AGTACTT17550.024.51654412-13
Produced by FastQC (version 0.11.2)