FastQCFastQC Report
Sun 14 Apr 2019
SRR6576778_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576778_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448169
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11840.2641860548141438No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8710.19434632917493178No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7060.15752986038748776No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6770.15105908708545213No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6550.1461502245804596No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6400.14280327287251013No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5220.1164739194366411No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5080.11335009784255493No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4940.11022627624846877No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4890.10911062567915228No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4870.10866436545142567No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4830.10777184499597249No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4740.10576367397120282No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4740.10576367397120282No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4600.10263985237711666No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC4560.10174733192166348No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4550.10152420180780017No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG4490.10018542112462041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10450.058.9234121
GGTATCA3750.043.870211
ATCAACG14950.041.1826743
TCAACGC15550.039.593634
CAACGCA15700.039.215355
AACGCAG16150.037.831656
GTACATG24600.036.3037451
TACATGG24350.036.097372
ACATGGG25200.034.3164023
TATCAAC18450.033.8834952
ACGCAGA18450.033.3702437
ATAGACT1001.4089583E-632.8989874
CGCAGAG19150.032.1504448
CCCTACG600.00415892631.3323679
CATGGGA10850.031.1879754
CGCACTA404.779355E-429.3757358-59
GTGTACG650.006147790728.9254111
GCAGAGT21400.028.7701429
GTACTAG856.8063405E-427.649291
CATGGGT7650.027.6462064
Produced by FastQC (version 0.11.2)