FastQCFastQC Report
Sun 14 Apr 2019
SRR6576778_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576778_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448169
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11450.25548398037347514No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7920.1767190501797313No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7890.17604965983814141No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7260.1619924626647537No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6850.15284412799635852No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5570.12428347342185649No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5480.12227530239708681No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5390.12026713137231713No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5210.11625078932277778No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5000.11156505693164856No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4990.11134192681778526No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4900.10933375579301559No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4740.10576367397120282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11850.057.2442471
ATCAACG16350.041.1088873
GGTATCA4500.039.779451
CAACGCA17050.039.145465
AACGCAG17350.039.0104066
TCAACGC17000.038.984114
GTACATG24600.034.660211
TATCAAC19700.034.356882
CTAACGC550.002714647734.1833573
GGTACCT4950.034.1795468
ACGCAGA19850.033.8566937
TACATGG25550.032.929082
ACATGGG25450.032.873783
CGCAGAG20500.032.7831888
TGGGTAC5800.030.794496
ATGGGTA5850.029.7278375
GGGTACC5750.029.4241317
CAATACT1154.1874137E-628.6099874
CATGGGT8350.028.1449814
GCAGAGT24150.027.828389
Produced by FastQC (version 0.11.2)