FastQCFastQC Report
Sun 14 Apr 2019
SRR6576779_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576779_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408556
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11100.27168858124712403No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7930.19409823867474718No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7150.17500660864116546No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7080.17329325722789532No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6870.16815320298808487No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5950.14563487012796286No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5350.13094900087136158No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5340.13070423638375156No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4810.11773171854042042No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4690.11479454468910015No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4670.11430501571388009No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4660.11406025122627009No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4660.11406025122627009No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4510.11038878391211977No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4280.10475920069708926No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC4120.10084296889532891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10550.053.0029031
CGCTAAA503.0076124E-546.9957165
CCGCTAA555.279932E-542.723384
GGACCGT555.279932E-542.723386
ATCAACG13150.042.5284423
TCAACGC13600.040.7756964
AACGCAG13650.040.6263356
CAACGCA14100.039.9963535
CCCGCTA608.8188266E-539.1630973
GTATATC608.82519E-539.1583021
GTACATG23850.037.2373281
TACATGG24300.036.9390222
GGTATCA4850.036.816881
ACATGGG24900.035.6714483
ATGGGTA4650.034.3624575
ACGCAGA16250.034.1219447
TATCAAC16450.033.9969022
CGCAGAG16750.033.103388
CATGGGT7550.032.990374
GGGTACC4850.032.9414187
Produced by FastQC (version 0.11.2)