FastQCFastQC Report
Sun 14 Apr 2019
SRR6576779_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576779_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408556
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11260.2756048130488844No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7580.1855314816083964No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7230.17696472454204565No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6130.15004063090494327No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6080.14881680846689316No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5840.14294246076425265No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5410.1324175877970217No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5310.12996994292092148No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4870.11920030546608054No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4800.11748695405281039No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4460.10916496147406966No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4440.1086754324988496No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4370.10696208108557945No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4150.10157726235815899No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4140.10133249787054896No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4140.10133249787054896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11650.059.7971651
ATCAACG16200.042.0687263
TCAACGC16700.040.8091774
CAACGCA16750.040.687365
AACGCAG17050.039.966566
GGTATCA4550.035.1732331
ACGCAGA19600.034.766837
TATCAAC20100.034.6076432
CGCAGAG20350.033.510118
GTACATG24450.033.497711
TACATGG24700.032.919672
ACATGGG24750.032.853173
ATGGGTA5500.032.4733625
CATGGGT7350.031.9734134
GTCCTAC902.5636273E-531.3800411
CTAGTAC753.2715005E-431.3339423
GGTACCT4800.030.373358
GCAGAGT22750.029.9749769
TGGGTAC5650.028.2802776
GGGTACC5550.027.0963137
Produced by FastQC (version 0.11.2)