FastQCFastQC Report
Sun 14 Apr 2019
SRR6576780_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576780_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452012
Sequences flagged as poor quality0
Sequence length100
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9910.21924196702742405No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5650.12499668150403086No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5160.11415626133819455No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5050.1117226976274966No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4780.1057494048830562No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4640.10265214197853155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10800.051.3650321
CAACGCA12950.039.915365
TCAACGC12950.039.915364
ATCAACG13200.039.1593863
AACGCAG13600.038.353166
GTACATG22100.036.5885731
TACATGG22100.035.95042
ACATGGG22550.035.2173963
TATCAAC15750.034.027782
CTAGTAC600.00416212231.3275093
CATGGGG12600.030.5816144
ACGCAGA17150.029.8661657
CGCAGAG17500.029.2688458
GGTATCA4850.028.1103081
TGGGTAC4350.027.0064726
ACCTAGT700.00881940426.8640371
GCATTAG909.494479E-426.1178111
GCAGAGT19800.026.1062559
TTAGGTA1101.01828395E-425.6315984
ATGGGGG4500.025.0620065
Produced by FastQC (version 0.11.2)