FastQCFastQC Report
Sun 14 Apr 2019
SRR6576780_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576780_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452012
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7870.1741104218472076No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6530.14446519118961446No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5550.12278435085794183No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4790.10597063794766512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9700.048.5718271
GTACATG21850.035.7942121
TCAACGC13450.035.289784
TACATGG22250.035.065172
ATCAACG13500.034.4628523
CAACGCA13700.034.3027735
ACATGGG23200.033.828153
AACGCAG13900.033.471126
TATTAGT1001.4098732E-632.896364
ATGGGGG4600.031.670415
CATGGGG13200.029.1937394
ATACCGT1452.6553607E-829.1691866
GTACTAG650.006077470728.9936451
TAGGTAT1154.1925978E-628.6055325
TATCAAC16200.028.43212
CATGGGT6950.028.3997364
ACGCAGA16300.028.2545437
CGCAGAG17150.027.1281958
ATGGGTA4700.026.9970135
TTAGTGC1057.417369E-526.8541726
Produced by FastQC (version 0.11.2)