FastQCFastQC Report
Sun 14 Apr 2019
SRR6576788_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576788_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751435
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA25610.3408145747802538No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC18270.24313480207868945No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13110.1744661880269085No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9630.1281547971547772No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9170.12203317652225408No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC8670.11537924105212027No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC8670.11537924105212027No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7560.1006075043084232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3750.061.507191
GTATCAA14250.055.8255351
TCAACGC17400.044.8299064
ATCAACG17750.043.9459343
CAACGCA18250.042.7419365
AACGCAG18400.042.3934976
TATCAAC21250.037.1500432
ACGCAGA22550.034.7999737
CTTAGGT5300.034.5778273
GTCTTAG5950.034.0182951
CGCAGAG23100.033.792728
TACATGG29850.033.3734052
GTACATG30450.033.081681
ACATGGG31050.031.6295993
GCAGAGT24700.031.6037169
TTAGGTA5700.031.3269234
TAGGTAT5750.031.0545145
AGGTATA5750.030.237296
GGTATAG6050.029.5146227
CATGGGG18900.029.3379124