FastQCFastQC Report
Sun 14 Apr 2019
SRR6576789_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576789_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1516438
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA22570.14883562664612732No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA21980.14494493015870083No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG20600.13584465701861864No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20220.13333878470468294No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT19330.12746976796941253No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA19260.12700815991158226No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA19190.12654655185375202No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA19170.12641466383722907No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA18420.12146886321761918No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18240.1202818710689128No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG17230.11362152623450482No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17040.11236859007753695No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT16980.11197292602796818No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC16710.11019243780490862No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC16340.10775250949923439No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG15380.10142188470613372No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGGAGCCGGG15310.10096027664830347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27900.048.527811
GTACTAG4400.039.532241
GGTATCA10900.037.9540861
CAACGCA36150.036.011815
ATCAACG36600.035.4406363
AACGCAG40800.032.598666
TCAACGC42000.030.8839824
ACCTAAG8450.030.0427321
GTACATG59650.028.1358591
TACATGG60050.027.948442
TATCAAC47800.027.931412
ACGCAGA46600.027.8353487
ACATGGG61850.027.2789883
CGCAGAG48250.026.8834658
CCTAAGA10250.025.684232
TAGTACT6900.025.2014834
TAGGCAT8300.024.9142655
CTAGGCA8750.024.7071844
GCAGAGT52800.024.6558159
CATGGGG39250.024.3069174
Produced by FastQC (version 0.11.2)