FastQCFastQC Report
Sun 14 Apr 2019
SRR6576790_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576790_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476459
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG23340.15808092198970647No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA22420.15184979738685597No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA20550.13918435933540993No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20190.13674609318646844No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT20160.1365429043407233No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA19740.13369826050029157No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA19720.13356280126979483No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA18430.12482568090275449No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT18310.12401292551977401No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG18300.12394519590452563No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA17780.12042325591161014No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA17120.11595310130521742No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16930.1146662386154983No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG16800.11378575361726943No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16690.1130407278495373No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG16530.11195705400556331No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC16410.11114429862258282No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT16390.11100883939208607No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16250.11006062477860881No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA15320.10376177056050997No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC15070.1020685301793006No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG14790.10017210095234612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26050.051.6996151
GGTATCA10400.040.750991
CAACGCA33100.040.460675
ATCAACG34450.038.738723
AACGCAG36750.037.210596
ACTACGC1055.122274E-835.8027883
TCAACGC40500.033.415944
TAGGTAT4550.033.0487255
CTTAGGT4450.032.7354163
ACGCAGA41400.032.3500867
TTAGGTA4800.032.306424
AGGTATA4700.031.9950646
TCTTAGG6650.031.7996082
CGCAGAG42550.031.4960238
GTACATG52200.030.7618791
TACATGG53050.030.3836272
CTACGCT1401.895205E-830.2086054
TATCAAC45600.029.9887942
ACATGGG54200.029.6512493
GTACTAG4450.029.6296841
Produced by FastQC (version 0.11.2)