FastQCFastQC Report
Sun 14 Apr 2019
SRR6576792_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576792_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916930
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA54820.5978646134383213No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC26860.2929340298605128No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21170.2308791292683193No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC19830.2162651456490681No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG19570.21342959658861635No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA15090.1645709050854482No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA13940.1520290534719117No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC12880.1404687380716085No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC12590.13730601027341235No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC11920.12999901846378678No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11460.12498227781837218No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA10570.11527597526528742No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT10070.10982299630288025No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT10060.10971393672363212No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT9840.10731462598017297No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG9810.10698744724242853No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT9450.10306130238949539No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT9380.10229788533475836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6200.073.5503161
GTATCAA27050.049.357771
ATCAACG31550.041.7104343
TCAACGC31550.041.7104344
CAACGCA32750.040.4691245
TATCAAC33000.040.0310362
AACGCAG33700.039.3283046
GTGCAAG5950.033.9747241
GTCTTAG9500.033.1555061
CGCAGAG40750.032.63968
GTACATG48650.032.565031
ACCTAAG18950.032.498741
TAGGTAT8150.032.29365
ACGCAGA41300.032.2049337
AAGGGTA5400.032.2028275
TACATGG49550.031.9735362
CTAAGAC20450.031.255873
CTTAGGT8300.031.143733
ACATGGG51050.030.7494053
TTAGGTA8450.030.5908834
Produced by FastQC (version 0.11.2)