FastQCFastQC Report
Sun 14 Apr 2019
SRR6576793_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576793_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923484
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA60980.6603254631374231No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC29510.3195507447882151No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22610.2448336950071685No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG19060.206392314322717No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC18530.2006531786148975No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG18470.20000346513854056No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA15560.16849236153522962No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13880.15030038419723568No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC13520.14640210333909412No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13370.14477781964820183No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA12980.1405546820518818No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC11860.12842669715988583No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT11780.12756041252474326No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC11550.12506984419870837No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA11370.1231207037696376No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT10620.114999285315176No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT10550.11424128625942626No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA10220.11066786213946316No Hit
TCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGA9960.10785243707524982No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT9820.10633643896375032No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT9550.10341272832014416No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA9390.101680159049859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7300.069.6507341
GTATCAA28950.053.0143971
TCAACGC32500.046.2716184
ATCAACG32900.045.7090453
CAACGCA33450.044.9574745
AACGCAG34550.043.659786
TATCAAC35700.041.994662
GGTAATC4600.040.8892338
AAGGGTA4650.039.4148375
ACGCAGA40800.036.8565257
CGCAGAG40950.036.7453968
TAGGTAT9800.034.5266535
GCAGAGT44050.034.372959
GTAATCA6200.033.370899
GTCTTAG11650.032.7328571
GTGCAAG6100.032.4148671
CTTAGGT10350.032.2378543
GTACATG49850.032.0154271
ATCCTAA1052.0379885E-631.3858241
TTAGGTA10650.031.329744
Produced by FastQC (version 0.11.2)