FastQCFastQC Report
Sun 14 Apr 2019
SRR6576796_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576796_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202592
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA42960.3572283866847609No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC29110.2420604826907214No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC24420.2030613874032091No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15420.1282230382374072No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15240.12672627125409117No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC15170.12614419520502382No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14410.11982451238657832No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13680.11375429073201884No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13610.11317221468295148No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12610.10485684255341794No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12270.10202961602937655No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12260.10194646230808121No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12260.10194646230808121No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT12070.10036654160346983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5650.062.4026261
GTATCAA24450.057.873231
TCAACGC28500.048.9771274
ATCAACG28850.048.3829463
CAACGCA29700.046.9982535
AACGCAG30850.045.2462926
TATCAAC36350.038.539132
ACGCAGA40000.035.0137027
CGCAGAG41600.034.0059478
GTACATG52900.033.5023881
GTCTTAG10300.032.4049381
TACATGG54000.032.3846552
CTTAGGT9150.032.3594553
ACATGGG56350.031.1931633
TAGGTAT9500.031.1672655
GCAGAGT44800.031.0524169
TTAGGTA9750.030.8501364
AGGTATA10200.029.0283346
GTACTAA650.00614789828.929221
GTATAGT12050.028.0819448
Produced by FastQC (version 0.11.2)