FastQCFastQC Report
Sun 14 Apr 2019
SRR6576796_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576796_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202592
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA47190.39240241079268784No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC28980.24097948431388203No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC24310.20214669646896039No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14940.1242316596152311No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13950.11599944120699289No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13430.11167544769963544No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG12660.10527261115989464No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12480.10377584417657858No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12060.1002833878821745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5700.066.173621
GTATCAA22250.055.730831
TCAACGC28100.043.6563454
ATCAACG28700.042.7472273
CAACGCA28850.042.684355
AACGCAG29800.041.4813356
CTTAGGT9250.038.1126253
GTACTAG757.2842686E-637.7189641
TTAGGTA9400.037.5013244
TAGGTAT9400.037.001315
TATCAAC34050.036.168752
GTCTTAG10800.035.7980881
AGGTATA10000.035.7212646
GGTATAG10000.035.7182967
GTATAGT11100.033.8722578
ACGCAGA37600.033.248417
TCTTAGG12250.032.9998322
CGCAGAG38600.032.3870548
ACCGTTC600.00416203131.3318358
GTACATG50500.031.2768611