FastQCFastQC Report
Sun 14 Apr 2019
SRR6576797_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576797_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136963
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA47060.41390968747443846No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC29770.2618378962200177No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23610.20765847261520384No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14180.12471821862276962No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13520.1189132803793967No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG12760.11222880603854303No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12630.11108540911181806No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT11680.10272981618575099No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11530.10141051203952987No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11470.10088279038104142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4800.062.7625271
GTATCAA21200.055.731351
ATCAACG25850.044.5390323
TCAACGC26400.043.7891434
CAACGCA27100.042.658065
AACGCAG28100.041.3053936
TATCAAC30200.037.3456082
ACGCAGA34500.033.3705337
TAGGTAT10400.032.985635
CTTAGGT10650.032.6525733
CGCAGAG35200.032.5963558
GTCTTAG12300.032.1466641
TTAGGTA10950.031.328824
ACCTAAG16700.030.1598381
GTACATG44100.029.886921
AGGTATA11600.029.5720256
TACATGG44350.029.5628192
GGTAATC3550.029.1417888
CCTAAGA18900.028.8424032
GCAGAGT40150.028.8131379