FastQCFastQC Report
Sun 14 Apr 2019
SRR6576802_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576802_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312115
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6040.19351841468689426No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4800.15378946862534643No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3710.11886644345834067No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3290.10540986495362287No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3260.10444868077471445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10450.039.7068021
TCAACGC11600.036.0551224
ATCAACG11800.035.4440163
CAACGCA11750.035.19495
AACGCAG11800.034.6475226
CGCAGAG13350.031.3287948
GGGCTAT902.590485E-531.3287936
ACGCAGA13400.030.86127
GTCCTCG804.683681E-429.469891
TATCAAC14450.029.2691142
AGCGGTC650.006146932528.9235294
ACCGTCG1503.6849087E-828.1959158
TACATGG16850.027.6102452
GCAGAGT14850.027.2149129
ATGGGTA3800.027.2065875
GTACATG16800.026.9439011
GTACAAC700.00868856626.9438971
ACATGGG17150.026.8532543
CATGGGT5100.026.7216194
GTAAGAC909.5101376E-426.1073273
Produced by FastQC (version 0.11.2)