FastQCFastQC Report
Sun 14 Apr 2019
SRR6576811_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576811_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953946
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA38650.40515920188354476No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC30260.3172087308925243No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC28880.3027425032444184No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG16530.17328024856752897No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14910.15629815524149165No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT13110.13742916265700575No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT12950.1357519188717181No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG12510.1311394984621771No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC12450.13051053204269422No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA11880.12453535105760705No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA11040.11572982118484695No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC10600.11111740077530594No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10550.11059326209240355No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10170.10660980810234541No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA9650.10115876580016059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5750.062.1423571
GTATCAA22600.047.6396981
ATCAACG22800.045.7615473
TCAACGC23500.044.598434
CAACGCA24350.043.234625
AACGCAG25450.041.5506026
TATCAAC28100.037.6459432
CTTAGGT9800.036.44773
GTATAGG2850.036.2927441
GTACATG41450.035.3893051
TTAGGTA10150.035.1908844
TAGGTAT10100.034.4344335
TACATGG42600.034.1028632
AGGTATA10100.033.9691056
ACGCAGA31500.033.570257
GTCTTAG10950.033.4905621
ACATGGG43300.032.996533
GGTAATC4400.032.0443278
CGCAGAG33350.031.708038
GGTATAG11000.031.1898147
Produced by FastQC (version 0.11.2)