FastQCFastQC Report
Sun 14 Apr 2019
SRR6576812_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576812_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878869
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA39510.44955505314216343No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC30590.34806097381976153No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC27840.31677075878202554No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG14460.16452963979842275No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT12250.1393836851680967No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12200.13881477216741062No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT12100.1376769461660384No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC11520.13107755535807952No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG10630.1209509039458668No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG10450.11890281714339679No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA10040.1142377305377707No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA9830.11184829593488906No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT9420.10718320932926295No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT9140.10399729652542074No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8860.10081138372157854No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG8860.10081138372157854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5200.059.755781
GTATCAA20200.050.8094521
TCAACGC22400.044.4770284
ATCAACG22600.044.0834273
CAACGCA22900.043.3006945
AACGCAG24150.041.251716
TATCAAC26950.037.491052
ACGCAGA28750.034.4860237
GGTATAT5250.032.2248652
GGGTATA5450.031.9627861
CGCAGAG31100.031.9055778
TACATGG37250.030.7830372
GTACATG37350.030.5045171
GGTAATC2850.029.7010778
GCAGAGT34400.028.844879
ACATGGG38950.028.5949153
ATACCGT3350.028.0548766
GTACTAA856.7471125E-427.6943041
GTCTTAG10200.026.7711621
CTTAGGT10050.026.653653
Produced by FastQC (version 0.11.2)