FastQCFastQC Report
Sun 14 Apr 2019
SRR6576813_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576813_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288124
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6230.21622634698949061No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4080.14160569754688954No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3310.11488109286279517No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA3300.11453402007469006No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3150.10932792825311323No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT3140.10898085546500812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTAG500.001687287637.6570851
CTCTCGT500.001700296837.59826394
GTATCAA9650.036.5839541
CAACGCA10900.032.3379555
AACGCAG11000.032.0439766
TCAACGC11150.031.1913854
GTACATG17500.030.9326061
ATCAACG11250.030.9141273
TACATGG17400.030.5693262
TACCGTC1252.3537541E-730.0786117
ACATGGG17400.029.441173
CATGGGT3700.027.9446564
ATACCGT1354.6162677E-727.8505676
ACGCAGA12800.027.537797
CATGGGG7800.027.1141344
CCGTCGT1406.3430707E-726.8559029
ATGGGGA4400.026.7033145
CGCAGAG13350.026.4032788
ATGGGTA2850.026.3847475
GTGCTAT909.416532E-426.1507511
Produced by FastQC (version 0.11.2)