FastQCFastQC Report
Sun 14 Apr 2019
SRR6576813_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576813_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288124
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5780.20060807152476015No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5070.17596590356929656No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4080.14160569754688954No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3280.1138398744984798No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA3260.11314572892226957No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3170.11002207382932348No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT2990.10377476364343129No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2910.10099818133859031No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG608.741883E-539.2164841
GTATCAA9650.038.5256271
GTATAAC651.4003481E-436.1998331
TATAGAG902.5893252E-531.3296322
ATCAACG13050.028.4487463
CAACGCA13150.028.2324055
GTTCCGG1354.619178E-727.8485694
TCAACGC13450.027.6026864
AACGCAG13500.027.5004566
CATGGGG7700.027.4642874
GTACATG17100.026.9699361
TACATGG17600.025.900352
ACATGGG17150.025.7578893
TAAGGTG1101.0165596E-425.6333355
GTATAGA1151.3619053E-424.5529331
TATCAAC14950.024.5188392
ACGCAGA15000.024.1238177
CGCAGAG15200.023.4972238
TCCGACC2052.9704097E-822.924128
CCGACCA2052.9704097E-822.924129
Produced by FastQC (version 0.11.2)