FastQCFastQC Report
Sun 14 Apr 2019
SRR6576816_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576816_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1369735
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA50960.37204276739661324No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC31690.23135862046308225No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC25600.18689746556815734No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16830.12287048224656594No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC15490.11308756803323271No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG14720.10746604270169048No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14170.10345066746487458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24150.058.1539541
GGTATCA6150.054.4080771
TCAACGC28750.047.247754
ATCAACG30200.044.9808733
CAACGCA30200.044.8235935
AACGCAG31300.043.398496
TATCAAC35750.037.603392
ACGCAGA39400.034.353417
TAGGTAT9800.034.052845
CGCAGAG41300.032.886788
TTAGGTA10450.032.3845144
CTTAGGT10650.031.7775123
TACATGG51300.031.061282
GTCTTAG11400.031.0053421
GTACATG51750.030.9634041
AGGTATA10800.030.8998076
CTAAGAC16700.030.397953
GGTAATC4650.030.3208838
ACCTAAG15650.029.8126751
GGTATAG11400.029.682557
Produced by FastQC (version 0.11.2)