FastQCFastQC Report
Sun 14 Apr 2019
SRR6576817_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576817_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302635
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA48860.3750858836128309No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC34380.2639265795867607No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC25250.1938378747692178No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16630.12766431118463728No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16300.12513098450448512No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG16000.12282796024980136No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC15280.11730070203856029No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT13730.10540174338936079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6750.065.457061
GTATCAA24700.054.8059841
TCAACGC30100.044.181014
ATCAACG30600.043.45913
CAACGCA31200.042.6233525
AACGCAG32300.041.1717766
TATCAAC35600.038.028422
TTACGCA500.001704655837.58856294
TAGGTAT10000.034.3035135
GTCTTAG11200.033.5741041
ACGCAGA40450.032.7589267
TTAGGTA10800.032.6327174
CTTAGGT10550.032.5151753
AGGTATA10800.031.3274086
GTACATG51800.031.3055821
TACATGG52000.031.0971722
CGCAGAG43100.030.7447458
GTATAGT12250.030.6868958
ACCTAAG17300.030.4301681
TATAGTA12750.029.8497419
Produced by FastQC (version 0.11.2)