FastQCFastQC Report
Sun 14 Apr 2019
SRR6576817_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576817_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302635
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA51140.39258886794842757No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC32090.24634682777600786No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC25220.1936075723437494No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17210.1321168247436926No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC14890.11430677050747139No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14200.10900981472169871No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG14000.10747446521857619No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT13230.1015633696315545No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13200.10133306720608612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6000.068.2456051
GTATCAA24600.056.6321871
ATCAACG29600.046.2034033
TCAACGC30000.045.43074
CAACGCA31150.043.602615
AACGCAG31650.042.908846
TATCAAC36200.037.782482
TTAAGAC4000.034.0730253
ACGCAGA39900.033.68217
ACTTAAG3800.033.4415861
CGCAGAG41500.032.520468
GTACATG51750.031.4682391
TAGGTAT8750.031.152485
TACATGG52150.031.0034582
GTCTTAG10650.030.4934231
CTTAGGT9100.029.9543063
GCAGAGT45650.029.461049
ACATGGG53950.029.4440753
AGGTATA9300.028.8014646
GGATAAT6050.028.7841241