FastQCFastQC Report
Sun 14 Apr 2019
SRR6576819_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576819_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321420
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA58080.43952717531140745No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC36150.27356934207140804No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC29210.22105008248702154No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG17690.1338711386236019No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC17220.13031435879584083No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC16200.12259538980793389No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15920.12047645714458688No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG14430.1092007083289189No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13560.10261688183923355No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT13420.10155741550756006No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13230.10011956834314602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7650.065.854391
GTATCAA28450.053.2888151
TCAACGC32400.045.5433164
ATCAACG32500.045.4031833
CAACGCA33650.043.851515
AACGCAG34950.042.3532686
TATCAAC39850.037.6185722
ACGCAGA44150.033.4212387
TAGGTAT11350.032.7092975
CTTAGGT11450.032.4236263
CGCAGAG46150.031.992238
GTCTTAG12850.031.510681
AGGTATA11800.031.4607186
GTACATG55500.031.218881
TACATGG56100.030.6590182
TTAGGTA12600.030.2102584
GCAGAGT49900.029.6822329
ACCTAAG18000.029.2959771
ACATGGG57600.029.044743
GGTATAG12850.028.8899977