FastQCFastQC Report
Sun 14 Apr 2019
SRR6576820_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576820_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136184
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA65560.5770192151975384No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC38870.3421100807615668No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC30930.2722270336494793No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG24940.2195066996190758No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC22810.20075973609908257No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC22210.19547890130471823No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA18140.15965723861628045No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA17700.15578462643374666No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT17450.15358427860276153No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15230.13404518986361363No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC15180.1336051202974166No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC14980.1318448420326285No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA14950.1315808002929103No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT14210.12506777071319433No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT13580.11952289417911184No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA13570.11943488026587243No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT13570.11943488026587243No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT13120.11547425417009921No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA12670.111513628074326No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT12250.10781704371827097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7800.056.6556661
GTATCAA29750.054.9923631
TCAACGC34650.045.8467864
ATCAACG35200.044.996913
CAACGCA35800.044.636625
AACGCAG36900.043.431456
TATCAAC37550.042.1919982
TAGGTAT13200.037.0300985
ACGCAGA44050.036.3818477
CGCAGAG44050.036.2751588
CTTAGGT13100.036.2364353
GTCTTAG14150.035.5498241
TTAGGTA13250.035.47154
GGTATAG13750.034.5219237
AGGTATA14100.033.998316
GCAGAGT47600.033.6684959
GTACATG55900.032.6309741
ACCTAAG20550.032.485281
TCTTAGG16300.032.3028262
TACATGG57050.032.055612
Produced by FastQC (version 0.11.2)