FastQCFastQC Report
Sun 14 Apr 2019
SRR6576820_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576820_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136184
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA66660.586700745653873No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC37980.33427684248325973No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC30660.2698506579920154No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC20970.18456517606303205No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG20710.18227681431880752No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC18520.16300176731937785No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT16500.14522295684501807No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG15660.13782978813290805No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT15260.13430923160333186No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14970.13175682811938913No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA14730.1296444942016434No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA14220.12515578462643373No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA14080.12392358984108208No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT13880.12216331157629398No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT13610.11978693591883005No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC13430.11820268548052076No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT13290.1169704906951691No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA13170.11591432373629623No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC12640.11124958633460778No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA12260.1079050576315104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5400.064.58581
GTATCAA25500.059.5133971
TCAACGC32650.045.346074
CAACGCA33850.043.738535
ATCAACG33950.043.4712523
TATCAAC34550.042.9884032
ACGTAAC555.1975356E-542.8456151
AACGCAG34550.042.444256
GTACTAG952.0658263E-839.688571
CGTAACA651.413203E-436.1550452
GTCTTAG13650.034.52761
ACGCAGA42750.033.970077
CGCAGAG43450.033.4227948
TAGGTAT12900.032.4274375
TTAGGTA13100.031.932364
CTTAGGT13450.031.450863
CTAGACG600.004160285531.3343723
GCAGAGT46550.030.9937179
AGGTATA14100.030.0009986
GGTATAG14350.029.1482287
Produced by FastQC (version 0.11.2)