FastQCFastQC Report
Sun 14 Apr 2019
SRR6576821_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576821_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100196
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA65050.59125828488742No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC41090.3734789073946824No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC29720.2701336852706245No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG25120.22832295336467323No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC22530.20478169344371366No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC21970.19969169129864134No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT17890.16260738995597146No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA17780.16160756810604657No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA17540.15942613861530128No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT15530.1411566666303095No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC15270.13879345134866877No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC14580.13252184156277608No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT14470.13152201971285116No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14080.12797719679039007No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA13680.12434148097248125No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT13390.12170558700449739No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT13020.11834254987293173No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA12890.11716094223211138No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA12830.11661558485942505No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT11710.10643558056928039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7000.064.471831
GTATCAA28350.050.741721
GTTAACG450.001012574241.7891962
ATCAACG32950.041.786053
TCAACGC33050.041.659624
TATCAAC33950.040.850632
CAACGCA33950.040.5533945
AACGCAG34900.039.4495056
GTCTTAG13650.033.4068761
CTTAGGT12500.033.0823
TTAGGTA12800.032.6737634
ACGCAGA43100.031.944037
TAGGTAT13300.031.7972985
GGTATAG13150.031.445347
CGCAGAG44000.031.2906288
GTACATG48700.030.8899941
ACCTAAG21050.030.8193721
AAGGGTA6450.030.5977125
GGTAATC6300.030.5803648
GTATAGT17400.030.5160688
Produced by FastQC (version 0.11.2)