FastQCFastQC Report
Sun 14 Apr 2019
SRR6576821_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576821_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100196
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA68440.6220709764441972No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC39880.36248086704550825No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC31880.2897665506873321No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC22030.20023704867132766No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG21030.19114775912655563No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC19400.17633221716857722No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG17400.15815363807903318No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT16560.1505186348614247No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT15030.1366120218579235No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA14890.13533952132165541No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14620.13288541314456698No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC14550.13224916287643293No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA14390.13079487654926938No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT14310.13006773338568764No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT14210.12915880443121044No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA14190.128977018640315No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT13810.12552308861330164No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA13160.11961505040919981No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC12870.11697915644121593No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA12750.11588844169584328No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC12260.111434689818905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7200.066.7088551
GTATCAA27450.055.0653571
TCAACGC32950.045.211644
ATCAACG33250.044.8037153
CAACGCA34100.043.686915
TATCAAC35100.042.9797672
AACGCAG35100.042.3083766
ACGCAGA42650.034.818857
CGCAGAG43500.034.2698828
ACCCGTA352.187754E-433.56592680-81
TTAGGTA11300.033.2703974
TAGGTAT11200.033.147865
CTTAGGT11350.033.123833
GTCTTAG12700.032.2575451
GCAGAGT48150.031.0579959
GTACATG51000.030.3767641
GGTATAG12250.030.3066127
TACATGG52200.029.4403822
AGGTATA12650.028.9768056
ACCGCGT650.006137134528.9393839
Produced by FastQC (version 0.11.2)