FastQCFastQC Report
Sun 14 Apr 2019
SRR6576824_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576824_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591457
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8490.14354382482581152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11950.050.3831061
CCGCTAA555.2789364E-542.7286954
ATCAACG14600.039.9191363
TCAACGC14950.038.6701854
CAACGCA15550.036.8758285
AACGCAG16300.034.887786
GTACATG26350.034.0953871
TATCAAC17250.034.061982
GGTATCA4800.032.3381841
TACATGG27450.032.0219882
ACATGGG27600.031.8452643
ACGCAGA18600.030.5737027
TGCCCGC1701.2187229E-1030.4359321
CATGGGT6900.029.9720134
AACGCCG1102.9644652E-629.9100885
ATAACGC1303.326022E-728.924043
ACGCCGC1303.3284778E-728.9215956
CGCAGAG20100.028.2920828
CCCGCTA856.8108254E-427.6479783
ATGGGTA4750.026.7166775
Produced by FastQC (version 0.11.2)