FastQCFastQC Report
Sun 14 Apr 2019
SRR6576824_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576824_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591457
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9560.1616347426778278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13600.042.2758331
ATCAACG15000.036.6566353
CAACGCA15200.036.177375
TCAACGC15450.035.588974
GGTATCA6000.034.5599171
AACGCAG15850.034.397236
TATCAAC18700.030.1653142
ACGCAGA18400.029.8856537
GTACATG23150.029.5180931
ACATGGG24000.028.7848593
CGCAGAG19300.028.492028
TACATGG24400.028.3201582
CATATAG1701.2428245E-724.880073
GCAGAGT22200.024.3466729
ATAGACG1000.001757748123.499838
CATGGGG11350.023.18734
GTACTAA1050.00229780522.4415041
CTAGTAC1050.002335410622.3788993
GCATAGA1354.0001827E-420.9454041
GTATAGG3001.8553692E-1020.421771
Produced by FastQC (version 0.11.2)