FastQCFastQC Report
Sun 14 Apr 2019
SRR6576825_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576825_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149099
Sequences flagged as poor quality0
Sequence length100
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3350.22468292879227894No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1850.1240786323181242No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT255.5395853E-575.210741
GTACACG200.002158051870.510071
TGGGTAC752.6066118E-950.1068576
ATAGACT405.675501E-446.9751786
GTATCAA5350.046.5673981
GGGTACC652.7786464E-643.36177
GTACATG9550.039.869571
TACATGG9800.038.852492
ACATGGG10000.038.0498923
CATGGGT1750.037.5801434
ATCAACG6200.037.125543
ATGGGTA906.167702E-736.536255
CAACGCA6200.036.3678785
AACGCAG6200.036.3678786
TCAACGC6400.035.965374
ATGGGAC1502.7284841E-1134.4484635
TATCAAC7000.034.247752
TAGCGCG550.002716490834.1637654
CATGGGA6200.033.3372234
GGTATCA2650.031.9290891
Produced by FastQC (version 0.11.2)