FastQCFastQC Report
Sun 14 Apr 2019
SRR6576825_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576825_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149099
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4570.3065077565912581No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2160.14487018692278286No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1840.1234079370082965No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.10529916364294864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA5600.047.2553981
GGTATCA2450.036.647041
ATCAACG7350.035.798793
CAACGCA7500.035.7092935
TCAACGC7400.035.5569044
GTATAGT550.002638368934.367561
CCGTCGT550.0027134534.171579
ATATACC550.0027134534.171573
AACGCAG8000.034.0647856
GTATAGC1051.9645122E-631.50361
GTACATG10550.031.3542921
TATCAAC8600.030.5954762
CATGGGG3100.030.3134924
TACATGG10650.030.0003952
ATAGCTG804.769541E-429.3661945
CGCAGAG9000.029.235688
ACATGGG10650.029.118033
ATGGGAG2101.8189894E-1229.0865155
ACGCAGA9200.028.6001197
GTATATA1406.0141065E-727.0030861
Produced by FastQC (version 0.11.2)