FastQCFastQC Report
Sun 14 Apr 2019
SRR6576831_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576831_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569286
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG53280.9359091915135802No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG33120.5817813893192525No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG32050.562985915690883No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT31330.5503384941839427No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG30250.5313673619235323No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC28920.5080047638621009No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC26870.47199474429372934No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG26340.462684836795565No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26150.4593473227867891No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT25190.4424840941108687No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC22160.3892595286024951No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG22040.387151625018005No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGGAGCCGGG21840.38363845237718824No Hit
GCATTTATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCC17560.30845655786370996No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAA17220.3024841643743215No Hit
GGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATG16830.29563347772472887No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC15740.2764866868322776No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT15590.2738518073516651No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT14880.2613800444767656No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT14390.25277277150676464No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA14330.2517188197145196No Hit
GCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCC14290.2510161851863562No Hit
GGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGC13660.2399496913677835No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT13570.23836876367941598No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCA12660.2223838281636998No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGGAGC12600.22132987637145476No Hit
GGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCC12390.2176410450985972No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT12210.2144791897218621No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA12140.21324957929757626No Hit
AATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCA11830.2078041617043103No Hit
CCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG11640.20446664769553444No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCG11380.19989952326247262No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11280.19814293694206428No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA11270.19796727831002345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11250.19761596104594178No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGG10940.1921705434526758No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGA10880.1911165916604308No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG10340.1816310255302256No Hit
GTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCC10330.18145536689818473No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG9900.17390204572042875No Hit
CGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGT9720.17074019034369367No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG9650.16951057991940782No Hit
TATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGG9530.1674026763349178No Hit
AAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCG9480.16652438317471357No Hit
AAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCG9330.16388950369410105No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT9140.16055198968532514No Hit
GTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCC8950.15721447567654923No Hit
CCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGT8930.15686315841246756No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG8860.1556335479881817No Hit
GCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGT8820.15493091346001833No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG8800.15457959619593667No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8790.1544039375638958No Hit
TGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAAT8700.1528230098755283No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT8580.15071510629103824No Hit
TCCCGGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGC8480.14895851997062987No Hit
GTACATGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCG8180.14368876100940478No Hit
ATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGGAGCCGGGA8180.14368876100940478No Hit
GTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGGAGCCG8130.14281046784920057No Hit
GGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATT8120.14263480921715974No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG7750.13613543983164875No Hit
ACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACC7610.13367621898307705No Hit
GTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGC7390.12981172907817864No Hit
TGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGA7360.12928475318205612No Hit
CCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCC7210.12664987370144357No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC7100.12471762874899435No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC7010.12313670106062682No Hit
TCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATG6980.12260972516450432No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA6950.12208274926838181No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6920.12155577337225928No Hit
CACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTT6870.1206774802120551No Hit
CCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTT6790.1192722111557284No Hit
GGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGCTCCCT6780.11909655252368757No Hit
CCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGA6750.11856957662756507No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGT6670.11716430757123836No Hit
CCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCG6580.11558337988287082No Hit
GTATCAACGCAGAGTACATGGGATCAGATCAAAACCAACCCGGTGAGCTC6500.11417811082654412No Hit
GTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT6410.11259718313817658No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT6330.1111919140818499No Hit
GGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCA6300.11066493818572738No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG6250.10978664502552321No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA6250.10978664502552321No Hit
CCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTTTGGTGACTCTA6020.10574649648858396No Hit
CTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTTTGGTGACTCTAGA6010.1055708378565431No Hit
CATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGA5990.10521952059246142No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG5900.1036385929040939No Hit
GATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGA5880.10328727564001222No Hit
TTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTG5870.10311161700797139No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTC5830.10240898247980805No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGAC5830.10240898247980805No Hit
GTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG5790.10170634795164468No Hit
GGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTTTGG5770.10135503068756302No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG5730.10065239615939968No Hit
CCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT5710.100301078895318No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAAG200.002158307270.533822
GGTATCA12950.062.4546551
GTATCAA41700.057.9606481
GTGCAAG2400.043.1040041
AAGGGTA2350.041.986935
ATCAACG56950.041.911453
CGTCACT3600.041.7978171
CAACGCA58600.040.731355
TCTAACG1305.456968E-1239.7883072
ACGCCTA1305.456968E-1239.7568556
CTAACGC1305.456968E-1239.7568553
TCAACGC60050.039.747834
AACGCAG60450.039.4848146
TAGTAGT608.8346525E-539.1544764
GGGTACC1600.038.1756177
GTACTAG1700.035.958421
GGAGATA1700.035.929998
GAGATAA1700.035.929999
CGCCTAC1451.8189894E-1135.6440777
AATAACT801.1600943E-535.2390334
Produced by FastQC (version 0.11.2)