FastQCFastQC Report
Sun 14 Apr 2019
SRR6576836_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576836_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137037
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2940.2145405985244861No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1770.12916219707086407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.10143245984661077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA5250.057.397751
TGCCCGC250.005189365256.500911
CTAGTAC250.00522703356.397813
GCCTAGT405.609441E-447.08411
ATCAACG6950.043.2788933
TCAACGC7100.043.02654
CAACGCA7050.042.6650125
AACGCAG7050.042.6650126
GTGTAAG450.001001968941.8525281
TATAAGC608.691336E-539.2367482
TATCAAC7800.038.6331022
CGCAGAG8600.034.9753888
ACGCAGA8750.034.375817
ACTATAG550.002709218334.180493
TGGGTAC1001.3996014E-632.8987246
ATGGGAG2200.032.044215
GTACATG10950.031.3893971
TACATGG10800.031.3893972
ATGGACC600.004149106331.3321194
CTGTGCG600.004149106331.3321199
Produced by FastQC (version 0.11.2)