FastQCFastQC Report
Sun 14 Apr 2019
SRR6576836_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576836_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137037
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4370.318891978078913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.13937841604822054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.12697300729000197No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1660.12113516787436969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA4450.047.6286931
ATACTAA450.001000357241.866211
ATTAAGG450.001011214241.7745323
GTACATG9700.036.417131
TACATGG9800.035.9665952
CTTAATC550.00270973334.1791653
ACATGGG10200.033.6346443
CATAGAG702.176004E-433.5688252
TCAACGC6550.033.0050744
ATCAACG6500.032.5359343
CAACGCA6650.032.508755
AACGCAG6700.032.266156
TATCAAC6600.032.0429652
ATGGGGG1501.0531949E-931.33095
TTACACT600.004149895631.3308984
CATGGGA7100.029.7864194
TAATCAT650.00613785228.920835
CATGGGG2750.027.343334
ATGGGAA3100.027.2882025
ATGGGAG2600.027.113285
Produced by FastQC (version 0.11.2)