FastQCFastQC Report
Sun 14 Apr 2019
SRR6576840_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576840_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280977
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA49770.3885315661405318No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC32970.25738167039689236No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC26610.20773206700822888No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18310.14293777327774035No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG16270.12701242879458413No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16230.12670016713805166No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16200.1264659708956523No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT14900.11631746705834686No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14780.11538068208874945No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14480.11303871966475587No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14300.11163354221035975No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13950.10890125271570059No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13860.10819866398850253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7450.053.6218221
GTATCAA23950.052.0019071
TCAACGC28750.041.838814
ATCAACG29650.040.72733
CAACGCA30050.040.028815
AACGCAG30800.039.054086
TATCAAC35250.034.798482
TAGGTAT9700.032.45495
ACGCAGA38950.030.8823017
GTACATG49200.030.3767261
CTTAGGT10400.030.2704353
CGCAGAG39850.030.1848378
AGGTATA10050.029.9220316
GTCTTAG11950.029.889911
TTAGGTA10600.029.6992934
TACATGG50450.029.530922
ACATGGG50300.028.9581573
GGATAAT5450.028.4574741
TCTTAGG12950.027.9447272
GCAGAGT43500.027.8681169
Produced by FastQC (version 0.11.2)