FastQCFastQC Report
Sun 14 Apr 2019
SRR6576841_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576841_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665808
Sequences flagged as poor quality0
Sequence length100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA24760.3718789801264028No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC15460.23219907240525794No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14000.21027082882752987No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG8890.13352197630548146No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC8280.12436017590656766No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC7690.1154987624059789No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT7510.11279528032105351No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT6870.10318289957465215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12500.051.135021
GGTATCA3450.050.4048271
TCAACGC14900.043.2139364
ATCAACG15350.041.3347133
CAACGCA16050.040.410445
AACGCAG16550.039.189586
TATCAAC16200.038.585682
TAGGTAT6000.032.1160555
CTTAGGT6100.031.5895583
TTAGGTA6050.031.0737844
ACCAGAT11050.030.19851794
ACGCAGA21600.029.5920267
GTACCGT650.0061529128.9225236
CTAAGAC11300.028.6985653
CGCAGAG22150.028.645058
ACCTAAG10350.028.1540491
GTGCTTA856.8138196E-427.6465281
GGTATAG6800.027.6465287
TATAGTA7500.027.5728059
GTACATG27900.027.2897991
Produced by FastQC (version 0.11.2)