FastQCFastQC Report
Sun 14 Apr 2019
SRR6576841_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576841_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665808
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA24520.3682743373465023No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17180.25803234566121164No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC12700.19074568043640208No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC7460.11204431307524092No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG7330.11009179823612814No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC6950.10438444716795232No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT6670.10017903059140172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3850.051.4153181
GTATCAA12250.050.0162541
ATCAACG14950.039.9261973
TCAACGC14900.039.429314
CAACGCA15400.038.149145
AGGGTAA2000.037.5997926
AAGGGTA2150.037.1625865
AACGCAG16100.036.490486
TATCAAC16650.035.5673682
TAGGTAT6650.033.924625
CTTAGGT7300.032.19163
TTAGGTA7200.031.9859334
AGGTATA7450.030.912586
GGTAATC2300.030.6497028
GGTATAG7450.030.2794367
ACGCAGA19600.029.7322947
GTCTTAG8300.029.527671
GTACATG25050.029.3508591
GTCTAGA650.00606941729.003511
CGCTTAG650.006152502728.9229152
Produced by FastQC (version 0.11.2)