FastQCFastQC Report
Sun 14 Apr 2019
SRR6576842_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576842_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521204
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA19770.37931404977705463No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12360.2371432299061404No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC11710.2246721053560602No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG7110.13641491623241572No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC6790.13027528568468394No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT6320.12125770331770287No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT6000.11511807276997107No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC5820.11166453058687194No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA5620.10782726149453957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9400.051.4863851
GGTATCA2050.048.1336171
ATCAACG11350.040.984763
TCAACGC11450.040.6268124
TATCAAC11800.039.4217832
CAACGCA11900.038.695655
AACGCAG12400.037.135346
GCCTAAC550.002719174134.1727831
GCGTAGT550.002719174134.1727831
TAGGTAT5800.034.0254865
TACATGG20400.033.8586922
CTTAGGT5850.033.7346733
GTACATG21100.032.735421
TTAGGTA6150.032.0890774
AGGTATA6400.031.5697776
TAAGGCG600.00416429831.3250529
ACTTAAG2100.031.325051
GTCTTAG6650.031.0895271
ACATGGG22100.031.0415653
GGTATAG6550.030.8468067