FastQCFastQC Report
Sun 14 Apr 2019
SRR6576842_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576842_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521204
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA19820.38027336705013776No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC16410.3148479290258709No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC12200.2340734146322745No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG6480.12432751859156874No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC6480.12432751859156874No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT6020.11550179967920429No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC5780.11089707676840548No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5550.10648421731222324No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT5320.10207135785604102No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT5260.1009201771283413No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT5220.10015272330987483No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC250.005237548656.3915373
GGTATCA3050.052.456041
GTATCAA10450.046.3807071
AGGGTAA2250.041.771516
ATCAACG11250.040.936083
TCAACGC11400.040.3974464
GGTAATC2200.038.4746178
CAACGCA11900.038.3051765
AACGCAG12400.037.1395876
TATCAAC13100.035.872482
AAGGGTA2400.035.244715
GGTACCT2200.034.199668
TTAGGTA4900.033.5663954
TATACCG851.75475E-533.1714944
GTCTTAG5700.033.0218621
TAGGTAT5150.031.9369545
GTAATCA2900.030.8091759
AGGTATA5450.030.1789596
GTACATG17550.030.0301361
CTTAGGT5350.029.864683