FastQCFastQC Report
Sun 14 Apr 2019
SRR6576847_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576847_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529197
Sequences flagged as poor quality0
Sequence length100
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13310.25151314160889043No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7060.1334096754138818No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7000.1322758821384097No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6830.12906346785790546No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6800.12849657122016941No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6430.12150484602142492No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6290.1188593283786567No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT5650.10676553344028783No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5560.1050648435270797No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA5430.10260829143022353No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA5390.10185242924657548No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5310.10034070487927936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12100.051.2789151
TACGTGT503.0151512E-546.979024
ACGGCGT450.001015687641.7591328
ATCAACG15750.038.4780583
CGCTAAA500.00170493237.5832185
CAACGCA16500.037.0137755
TCAACGC16450.036.8406944
AACGCAG17100.035.7150466
GGTATCA5000.033.8440861
GTACATG26300.033.6010131
TACATGG26350.033.3588642
GGTACCT5250.033.1090288
ACATGGG26650.032.6120833
GGGTACC5650.031.596517
CTAGTAC753.2817508E-431.319353
CCCGCTA600.00416806731.319353
TGGGTAC5400.030.4493666
ACGCAGA20350.030.0111717
ATGGGTA5950.030.0034125
TATCAAC20600.029.2074092