FastQCFastQC Report
Sun 14 Apr 2019
SRR6576847_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576847_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529197
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11660.22033382653340816No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7910.14947174681640296No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7150.13511036532708992No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6850.1294413989497295No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6480.12244967375098499No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6220.11753656955727262No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6210.11734760401136062No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5740.10846622335349596No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA5610.10600967125663978No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5530.10449794688934366No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA5410.1022303603383995No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT5320.10052967042519137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAA250.005237753556.39115
GTATCAA13950.048.6694341
TCAACGC17950.038.2223784
AACGCAG18000.037.5940676
CAACGCA18100.037.386365
ATCAACG18600.036.6340033
GTACATG28500.034.244711
TACATGG28350.033.9837382
TAGGTAT1554.1836756E-1133.3495755
ACATGGG29150.032.564333
ACGCAGA21350.031.255027
GGTATCA5650.030.8760171
CGCAGAG21950.030.400678
TATCAAC23300.029.8521862
CATGGGG15250.029.5822164
GGTACCT4500.029.239838
TTAGGTA1755.5479177E-926.8529054
GGGCGTA700.00883859126.8529039
TGGGTAC4800.026.4333256
GCAGAGT25300.026.189549
Produced by FastQC (version 0.11.2)